TABLE 1.
Name | Description/genotype | References |
Clinical isolates | ||
PA KR794 | Urine isolate | Paulsson et al., 2015 |
PA KR796 | Airway isolate from a patient with CF | Paulsson et al., 2015 |
PA KR799 | Blood isolate | Paulsson et al., 2015 |
PA KR801 | Airway isolate from a patient with CF | Paulsson et al., 2015 |
Laboratory strains | ||
E. coli BL21(DE3) | E. coli laboratory strain | Studier and Moffatt, 1986 |
E. coli DH5α | E. coli laboratory strain | Taylor et al., 1993 |
E. coli pET16b-pba | E. coli BL21(DE3) for soluble expression of Pba | This study |
E. coli pET16b-oprG | E. coli BL21(DE3) for soluble expression of OprG | Paulsson et al., 2019 |
PAO1 | P. aeruginosa reference strain | Held et al., 2012 |
PW2884 | PAO1 mutant with ISlacZ/hah transposon insert in pba | Held et al., 2012 |
PW2884 + pba | PW2884 complemented with pUCP22Not-pba | This study |
Plasmids | ||
pUCP22Not-pba | Complementation plasmid with PA1041 insert. Gentamicinr | Herrero et al., 1990 |
pET16b-pba | 6 × His-tagged Pba expression vector. Ampicillinr | This study |
pMRP9 | GFP expression vector. Carbenicillinr | Davies et al., 1998 |