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. 2021 Feb 25;12:639582. doi: 10.3389/fmicb.2021.639582

TABLE 1.

Clinical Pseudomonas aeruginosa isolates, laboratory strains, and plasmids used in this study.

Name Description/genotype References
Clinical isolates
PA KR794 Urine isolate Paulsson et al., 2015
PA KR796 Airway isolate from a patient with CF Paulsson et al., 2015
PA KR799 Blood isolate Paulsson et al., 2015
PA KR801 Airway isolate from a patient with CF Paulsson et al., 2015
Laboratory strains
E. coli BL21(DE3) E. coli laboratory strain Studier and Moffatt, 1986
E. coli DH5α E. coli laboratory strain Taylor et al., 1993
E. coli pET16b-pba E. coli BL21(DE3) for soluble expression of Pba This study
E. coli pET16b-oprG E. coli BL21(DE3) for soluble expression of OprG Paulsson et al., 2019
PAO1 P. aeruginosa reference strain Held et al., 2012
PW2884 PAO1 mutant with ISlacZ/hah transposon insert in pba Held et al., 2012
PW2884 + pba PW2884 complemented with pUCP22Not-pba This study
Plasmids
pUCP22Not-pba Complementation plasmid with PA1041 insert. Gentamicinr Herrero et al., 1990
pET16b-pba 6 × His-tagged Pba expression vector. Ampicillinr This study
pMRP9 GFP expression vector. Carbenicillinr Davies et al., 1998