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. 2021 Feb 25;10:614365. doi: 10.3389/fonc.2020.614365

Figure 1.

Figure 1

Downregulation of PD-L1 in MDA-MB-231 cells. (A) Relative fold change of PD-L1 messenger RNA (mRNA) expression in MDA-MB-231 cells. Fold changes were normalized to untreated cells. Values are represented in box and whisker format with minimum to maximum values from 4–7 independent experiments. (B) Representative immunoblot assays of PD-L1 (top) and GAPDH (bottom) following PD-L1 siRNA dextran NP incubation. MDA-MB-231 cells were untreated (control) or transfected with either 100 nM scrambled siRNA/dextran, or 100 nM PD-L1 siRNA/dextran (N/P = 15). ***p ≤ 0.001 compared to untreated cells. ###p ≤ 0.001 compared to cells transfected with scrambled siRNA.