Figure 7.

CU-ACC2 patients shows a partial response to treatment with anti-PD-1 pembrolizumab. A, Pre-pembrolizumab multispectral fluorescence immunohistochemistry of ACC-M2B lesion shows evidence of CD4⁺ and CD8⁺ immune cell infiltration in the tumor adjacent stroma. Regions on the tumor border or those away from the tumor border were chosen for quantification and segmented into tumor or stroma areas using inForm software. The density of cells for each indicated phenotype was determined for central tumor regions (black), intratumor stroma (red), tumor regions near the tumor border (white), or tumor adjacent stroma (gray). B, Changes in representative target lesion 2 over 16-month period using serial contrast-enhance CT.