FIGURE 3.

Fisetin ameliorates the expression of JNK and its associated inflammatory mediators in d -galactose injected mice (A,B and C). Immunofluorescence results of p-JNK, Iba-1, and GFAP in the experimental mice’s brains. (D). Western blot results of p-JNK, JNK p-NF-Kβ, NF-Kβ, IL-1β, Iba-1, GFAP, TNF-α, and NOS-2 in the experimental mice’s brains. β- Actin was used as a loading control. n = 10 mice per group for western blot and immunofluorescence analysis, number of experiments = 3. *p < 0.05, **p < 0.01 represent control vs. D-gal treated mice, #p < 0.05, ##p < 0.01 represent D-gal vs. D-gal + fisetin. LPO: Lipid peroxidation, ROS: Reactive Oxygen Species, Con: Control, D-gal: d-galactose, Fis: Fisetin, Hippo: Hippocampus, DG: Dentate Gyrus.