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. 2021 Feb 25;12:612078. doi: 10.3389/fphar.2021.612078

FIGURE 4.

FIGURE 4

Effects of Fisetin against the apoptotic cell death in the D-gal-treated mice Brains. (A). Western blots results of Bax, Bcl2, cleaved caspase-3, and cleaved PARP-1 in the mice brains (B and C) Immunofluorescence results of caspase-3 and flourojade-B in the experimental mice brains. β- Actin was used as a loading control. Values are the means ± SEM from three independent experiments. Magnifications ×10, Scale bar 50 µm n = 10 mice per group for western blot and immunofluorescence analysis, number of experiments = 3. *Significantly different from normal mice # significantly different from D-gal-treated mice, respectively; *p < 0.05, **p < 0.01 represent control vs. D-gal treated mice, #p < 0.05, ##p < 0.01 represent D-gal vs. D-gal + fisetin. Con: Control, D-gal: d-galactose, Fis: Fisetin, Hippo: Hippocampus, DG: Dentate Gyrus.