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. 2021 Feb 25;12:612078. doi: 10.3389/fphar.2021.612078

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Copyright © 2021 Ahmad, Khan, Ali, Jo, Park, Ikram and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of Fisetin against the synaptic dysfunction in D-gal-treated mice. (A). Western blot resulted in SNAP-25 and PSD-95 in the experimental mice, β- Actin was used as a loading control. (B). Immunofluorescence images of PSD-95 in the cortex and hippocampus of the treated groups. Magnification ×10. Scale bar 50 µm n = 10 mice per group for western blot and immunofluorescence analysis, number of experiments = 3. *p < 0.05, **p < 0.01 represent control vs. D-gal treated mice, #p < 0.05, ##p < 0.01 represent D-gal vs. D-gal + Fis. Con: Control, D-gal: d-galactose, Fis: Fisetin, Hippo: Hippocampus, DG: Dentate Gyrus.