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. 2021 Feb 25;12:642829. doi: 10.3389/fmicb.2021.642829

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Copyright © 2021 Bjerketorp, Levenfors, Nord, Guss, Öberg and Broberg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Top: Heat map generated by hierarchical clustering (Euclidean, complete) of major compounds (rows) produced by 33 strains of Pedobacter (columns) as analyzed by UHPLC-MS. Numbers indicate clustering of compounds discussed in the text. [UP-strains are from this work. P. cry., Pedobacter cryoconitis A37 (LMG 21415^T); P. lus., P. lusitanus NL19 (LMG 29220^T); P. har., P. hartonius WB 3.3-3 (DSM 19033^T); P. wes., P. westerhofensis WB 3.3-22 (DSM 19036^T); P. him., P. himalayensis HHS22 (JCM 12171^T).] Bottom: Chromatograms (A210) from HPLC-fractionation of the indicated Pedobacter isolates, with data from bioassays against a panel of bacterial pathogens under each chromatogram. Bacteria are shown in rows (from top to bottom: E. coli, A. baumannii, E. cloacae, K. pneumoniae, P. aeruginosa, and S. aureus) and HPLC fractions in columns. Activity grading of fractions is ranging from red (full inhibition) to white (no visible inhibition). The presence of peptides from clusters 1–14 is indicated in each chromatogram.