TABLE 1.
Ingredients and proximate chemical composition of the experimental diet without supplementation (g kg–1 on dry matter basis).
| Feed ingredients | Composition and chemical analysis |
| Fish meal (65%) | 160 |
| Yellow corn | 280 |
| Soybean meal (40%) | 400 |
| Wheat bran | 100.5 |
| Soybean oil | 20.5 |
| Distiller’s dried grain with solubles | 9 |
| Vitamins and Minerals mixture1 | 30 |
| Total | 1000 |
| Chemical analysis (on dry matter basis) | |
| Dry matter (Dm) % | 922.8 |
| Crude protein (CP) % | 301.8 |
| Ether extract (EE) % | 44.4 |
| Crude fiber (CF) % | 93.3 |
| Ash % | 101.2 |
| NFE2 % | 459.3 |
| Me (Kcal/Kg diet)3 | 2610 |
| P/E ratio4 | 115.6 |
1Vitamin and mineral mixture/kg premix: vitamin D3, 0.8 million IU; A, 4.8 million IU; E, 4 g; K, 0.8 g; BI, 0.4 g; Riboflavin, 1.6 g; B6, 0.6 g; B12, 4 mg; pantothenic acid, 4 g; Nicotinic acid, 8 g; Folic acid, 0.4 g Biotin, 20 mg, Mn, 22 g; Zn, 22 g; Fe, 12 g; Cu, 4 g; I, 0.4 g; Selenium, 0.4 g and Co, 4.8 mg.
2Nitrogen free extract (NFE) = 100 – (CP + EE + CF+ Ash).
3Metabolizable energy was calculated from ingredients based on NRC (2011) values for tilapia.
4Protein to energy ration in mg protein/Kcal ME.