Figure 2.

Micro ribonucleic acid (miR)-15 reduced angiogenesis in the retinal endothelial cells. (A) The mimic of miR-15b or negative control (NC) was labeled with cyanine (Cy) 3 and added at different doses (0, 25, 50, and 100 pM) into the culture medium of human retinal microvascular endothelial cells. The tube formation assay was used to detect the effect of miR-15b on angiogenesis. The images were taken by fluorescent microscope with bright light or Cy3 channel. (B and C) The branch points and tube lengths were quantified. (mean ± SD, n = 3; *P < 0.05, **P < 0.01, vs 0 pM treatment group; #P < 0.05, ##P < 0.001 vs NC in the respective group). SD, standard deviation.