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. 2020 Aug 14;105(11):3404–3415. doi: 10.1210/clinem/dgaa538

Figure 3.

Figure 3.

Micro ribonucleic acid (RNA) (miR)-15b decreased messenger RNA (mRNA) and protein expression of vascular endothelial growth factor (VEGF) in the retinal endothelial cells. The mimic of miR-15b or negative control (NC) added at different doses (0, 25, 50, and 100pM) into the culture medium of human retinal microvascular endothelial cells. (A) After treatment with miR-15b and NC for 48 hours, the mRNA levels of VEGF were detected by real time polymerase chain reaction, while β-actin acted as an internal control (mean ± SD, n = 3; *P < 0.05, **P < 0.01, v. 0 pM treatment group; #P < 0.05, ##P < 0.001 vs NC in the respective group). (B and C) After treatment with miR-15b and NC for 48 hours, the protein levels of VEGF were detected by Western blot. Relative levels of VEGF were quantified by densitometry in 3 independent experiments in comparison with β-actin (mean ± SD, n = 3; **P < 0.01, vs 0 pM treatment group; ##P < 0.001 vs NC in the respective group). SD, standard deviation.