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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Neurobiol Aging. 2020 Jul 25;95:154–160. doi: 10.1016/j.neurobiolaging.2020.07.016

Fig. 3.

Fig. 3.

Suppression of eEF2K activity improves hippocampal synaptic morphology of aged mice. (A) Representative TEM images for postsynaptic densities (PSDs) in area CA1 of hippocampus. Arrows indicate PSDs. (n = 3. Original magnification, x11,000. Scale bar: 250 nm.) (B) Quantification of PSDs per μm2. (C) Quantification of average active zone length in nm. (****P < 0.0001, unpaired t test.) (D) Quantification of average PSD size in nm2. (****P < 0.0001, unpaired t test.) (E) Representative images from Golgi-Cox stain of dendritic spines in hippocampal area CA1. (n = 3. Original magnification, x100. Scale bar: 10 μm.) (F) Quantification of total spines in area CA1 per 100 μm. (WT, n = 153 dendrites; eEF2K KO, n = 132 dendrites. Unpaired t test.) (G) Mature spine density per 100 μm in which branched, mushroom and stubby spines were categorized as mature. (H) Immature spine density per 100 μm in which thin, long thin and filopodial spines were categorized as immature. (I) Stubby spine density per 100 μm.