Figure 3.

CircBCL11B is exclusively expressed in AML. (A) Schematic diagram of the BCL11B gene consisting of 4 exons and circBCL11B resulting from a circularization of exon 2. The backsplice site is indicated by a red line. (B) Normalized circular read counts of circBCL11B in 61 AML patients and 16 healthy HSPC samples, as detected in the ribosomal RNA–depleted RNA-seq data. (C) CLP of BCL11B circRNA junction counts to BCL11B linear mRNA junction counts. (D) CircBCL11B-derived PCR products were generated using different AML cell lines and the CML cell line K562 and divergent primers (red arrows) specific for exon 2 of BCL11B. PCR products were pooled and sequenced on an Oxford Nanopore MinION. Nanopore reads were aligned to BCL11B using the National Center for Biotechnology Information’s Basic Local Alignment Search Tool. A sequencing result of circBCL11B is shown; the fusion site is indicated by a red line. NTC, no template control.