Table 3.
Proportion of released N-glycans from different batches of Expi293F-derived CSL040 or HuCR1(1971), showing asialo (neutral), monosialo, disialo, trisialo, and four or more (tetrasialo+) sialic acids
| N-glycan type | CSL040 (%) | HuCR1(1971) (%) | CSL040 (%) | HuCR1(1971) (%) |
|---|---|---|---|---|
| Asialo | 24.1 | 28.0 | 74.5 | 77.6 |
| Monosialo | 22.8 | 25.9 | 21.1 | 17.6 |
| Disialo | 41.9 | 37.3 | 3.7 | 4.3 |
| Trisialo | 9.1 | 6.3 | 0.6 | 0.4 |
| Tetrasialo+ | 2.1 | 2.5 | 0.1 | 0.1 |
Each number is expressed as a percentage of the total glycan content measured. The batches of CSL040 and HuCR1(1971) with the lower asialo content were generated by coexpression of B4GALT1 and ST3GAL3.
HuCR1, human complement receptor 1.