Table 1.
Biological characteristics of the mouse models
| Variables | N (each group) | CKD-bMPOWT | CKD-bMPOKO | p-value |
|---|---|---|---|---|
| Bodyweight (gm) | ||||
| Baseline | 10 | 22.5 ± 1.4 | 22.8 ± 1.8 | 0.67 |
| Post-BMT | 10 | 24.2 ± 1.4 | 24.2 ± 1.7 | 0.99 |
| 16 weeks (HFD) | 10 | 29.4 ± 2.9a | 28.1 ± 2.4a | 0.41 |
| Systolic BP (mmHg) | 5 | 161.96 ± 14.6 | 158.9 ± 13.8 | 0.84 |
| Diastolic BP(mmHg) | 5 | 139.56 ± 14.5 | 132.4 ± 12.6 | 0.46 |
| Creatinine (mg/dl) | ||||
| Pre-BMT | 5 | 0.25 ± 0.04 | 0.31 ± 0.12 | 0.36 |
| Post-BMT | 5 | 0.32 ± 0.07 | 0.33 ± 0.05 | 0.79 |
| 16 weeks (HFD) | 5 | 0.22 ± 0.02a | 0.24 ± 0.05a | 0.56 |
| Hematocrit (%) | ||||
| Baseline | 10 | 50.9 ± 1.1 | 51.2 ± 1.8 | 0.65 |
| Post-BMT | 10 | 51.6 ± 2.5 | 52.4 ± 4.2 | 0.63 |
| 16 weeks (HFD) | 10 | 51.3 ± 4.5 | 51.5 ± 3.0 | 0.94 |
| Serum calcium (mg/dl) | 8 | 12.8 ± 2.5 | 12.5 ± 2.6 | 0.8 |
| Serum phosphorus (mg/dl) | 8 | 20.48 ± 4.33 | 24.6 ± 4.9 | 0.1 |
| Intact parathyroid hormone (pg/ml) | 5 | 17.9 ± 14.0 | 32.3 ± 37.1 | 0.44 |
BMT, bone marrow transplant; BUN, blood urea nitrogen; CKD-bMPOKO, irradiated 5/6 nephrectomized LDLr−/− mice with bone marrow from MPO knockout mice; CKD-bMPOWT, irradiated control 5/6 nephrectomized LDLr−/− mice with bone marrow from WT donor mice; HFD, high-fat/high-cholesterol diet; iPTH; intact parathyroid hormone.
a
p-value < 0.05 as compared with mice in the same group at previous time points.