Figure 5.

Effect of cecr2 gene structure on its reprogramming activity.A, schematic illustration of Cecr2 structure and mutants. B, effect of various Cecr2 mutants on OKS-induced reprogramming (n = 6 wells from three independent experiments; mean ± SD, two-tailed, unpaired t test; ∗∗∗p < 0.001.). C, IP and immunoblot analysis of the interaction between CECR2-HA and SNF2L-FlAG. D, IP and immunoblot analysis of CECR2-HA/CECR2 ΔDDT-HA and SNF2L-FLAG indicates the DDT domain is essential for the interaction between CECR2 and SNF2L. SNF2L with a molecular weight of 123 kDa is indicated. Asterisks indicate non-specific bands. E, heatmap of IP-MS analysis showing Top 20 proteins enriched in CECR2. NOTE that the deletion of DDT domain disrupts the interaction between CECE2 and SMARCA1(SNF2L). F, heatmap of GO analysis for top 100 proteins enriched in OKS+Cecr2-3×FLAG VS OKS+DsRed-3×FLAG (Control) or top 100 proteins enriched in OKS+Cecr2-3×FLAG VS OKS+Cecr2 ΔDDT-3×FLAG reprogramming in day 3 through FLAG-IP MS.