Figure 3.

Comprehensive scanning mutagenesis of positions P1’–P4’ in prelamin A to all other 19 amino acids. Western blots for all possible variants of residues L647 (A), L648 (B), G649 (C), and N650 (D), are shown. For each residue, WT is indicated in red. Annotation of uncleaved and mature lamin A, quantification of cleavage, and statistical analysis are performed as explained in the legend for Figure 2.