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. 2020 Dec 17;296:100182. doi: 10.1074/jbc.RA120.013908

Figure 2.

Figure 2

ATP13A3 is downregulated in CHO-MG cells.A, ATP13A1-3 mRNA levels were measured with quantitative RT-PCR using SYBR Green master mix (n = 4). B, protein expression of ATP13A3 was checked via proteomic analysis (n = 4). C, ATP13A3 topology model with the identified mutations in CHO-MG cells. Data represent the mean ± SD (A and B) and individual data points (representing replicates) are overlaid on bar graph plots (∗∗p < 0.01, ∗∗∗∗p < 0.0001). Analyses were performed using one-way ANOVA and Bonferroni post hoc corrections (A) and unpaired t-test (B). A1-A3, ATP13A1-3; FKBPA1, FKBP prolyl isomerase 1A.