Figure 6.

ABCA13 deletion impairs vesicular cholesterol accumulation and synaptic vesicle endocytosis in neurons.A, primary mouse cortical neurons were treated with 5 mM MβCD for 5 min at 37 °C. Then, the cells were labeled with anti-βIII-tubulin antibody (magenta) and the fluorescent probe EGFP-D4 (green) after fixation and permeabilization. B, the relative fluorescence intensities of EGFP-D4 signals in individual cells were quantified using ImageJ and shown as means + S.E.M. (n = 30). C, primary mouse cortical neurons were stained with FM 4-64 for 3 min in high K+ buffer. Then, the cells were washed for 10 min in Ca²⁺-free buffer and observed using fluorescence microscopy. D, the relative fluorescence intensities of FM 4-64 signals in individual cells were quantified using ImageJ and shown as means + S.E.M. (n = 30). Scale bars represent 20 μm. ∗p < 0.05, ∗∗p < 0.01.