Table 1.
Kinetic parameters for recombinant RhGmaS with different substratesa
| Substrate | Km (μM) | Vmax (μM min−1) | kcat (s−1) |
|---|---|---|---|
| MMA | 26.94 ± 1.73 | 3.58 ± 0.11 | 4.18 ± 0.13 |
| Ethylamine | 58.10 ± 4.49 | 3.51 ± 0.11 | 4.09 ± 0.13 |
| Hydroxylamine | 211.03 ± 9.87 | 3.56 ± 0.05 | 4.15 ± 0.06 |
| Propylamine | 1.79 × 103 ± 0.12 × 103 | 3.63 ± 0.08 | 4.23 ± 0.09 |
| NH4Cl | 8.72 × 103 ± 0.97 × 103 | 3.65 ± 0.17 | 4.26 ± 0.20 |
| DMA | 16.07 × 103 ± 1.15 × 103 | 2.20 ± 0.09 | 2.57 ± 0.10 |
| TMA | 22.91 × 103 ± 1.50 × 103 | 2.13 ± 0.06 | 2.48 ± 0.07 |
DMA, dimethylamine; MMA, monomethylamine; TMA, trimethylamine.
a
The experiments were performed at pH 8.0, 30 °C. The data shown in the table are from triplicate experiments (means ± SDs).