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. 2020 Dec 29;296:100227. doi: 10.1074/jbc.RA120.014288

Figure 2.

Figure 2

The nonspecific kinase inhibitor staurosporine reduces the hyperpolarized shift of the I848T mutation. A, voltage dependence of activation using 500 nM staurosporine, incubated for 20 min. Staurosporine reduces the hyperpolarized shift of the I848T mutation from −9.77 ± 1.45 to −6.67 ± 1.54 mV. B, V1/2 of channel activation (ANOVA F = 25.67; WT versus I848T p <0.0001, mean diff. 9.77 ± 1.45 mV, 95% confidence interval [CI] 5.88–13.65 mV; WT+S versus I848T + S p = 0.0002, mean diff. 6.67 ± 1.54 mV, 95% CI 2.53–10.80 mV; I848T versus I848T + S p = 0.0064, mean diff. −5.14 ± 1.529 mV, 95% CI −9.247 to −1.032 mV). C, staurosporine does not significantly affect current density (ANOVA F = 4.8; WT versus I848T p = 0.0213, mean diff. −162.9 ± 54.68 pA/pF, 95% CI −309.8 to −15.99 pA/pF). D, current–voltage relationship with or without staurosporine. Data for WT and I848T are the same as for Figure 1 and only presented for comparison. All are one-way ANOVA with Bonferroni multiple comparisons test. See Tables 1 and 2 for all values. All data are presented as mean ± SD. WT + S = WT + staurosporine, I848T + S = I848T mutant + staurosporine.