Figure 3.

Various cysteine-to-serine mutants result in an increase in the DNA dissociation constant. Representative electromobility gel shifts for A, WT OGG1 and B, C255S binding to their DNA substrate containing 8-oxoG lesion with either templating cytosine (left gel) or templating adenosine (right gel). Fraction DNA bound was plotted as a function of OGG1 concentration and fitted to a sigmoidal equation for the templating C, cytosine and D, adenosine in order to extract the apparent binding constants for each of the mutants relative to the WT (E).