Table 3.
Functional effects of low concentrations of antagonist mixtures on ORco function
| Compound/mixtures | Compound conc. (μM) | % Inhibition (±SD) | Predicted additive sum of % inhibition | Blends versus single compound at 25 μM Pr(<[t]) | Predicted versus actual blend inhibition Pr(<[t]) | Blend versus single compound at 50/75 μM Pr(<[t])a | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| OA | 100 | |||||||||
| CRV | +25 | 31.75 ± 1.21 | ||||||||
| +50 | 43.50 ± 2.51 | |||||||||
| +75 | 47.20 ± 1.82 | |||||||||
| CA | +25 | 22.08 ± 0.78 | ||||||||
| +50 | 27.46 ± 2.90 | |||||||||
| +75 | 39.41 ± 3.09 | |||||||||
| OCT | +25 | 22.70 ± 1.68 | ||||||||
| +50 | 30.68 ± 3.58 | |||||||||
| +75 | 39.93 ± 2.29 | |||||||||
| CRV+CA | +25+25 | 49.61 ± 4.46 | 53.83 | CRV | CA | 0.232882 | CRV | CA | ||
| 0.00001 | 0.000001 | 0.023823 | 0.001242 | |||||||
| CRV+OCT | +25+25 | 44.38 ± 3.15 | 54.45 | CRV | OCT | 0.021612 | CRV | OCT | ||
| 0.000267 | 0.000004 | 0.708954 | 0.000446 | |||||||
| CA+OCT | +25+25 | 39.96 ± 1.58 | 44.78 | CA | OCT | 0.391426 | CA | OCT | ||
| 0.000447 | 0.00025 | 0.051099 | 0.021817 | |||||||
| CRV+CA+OCT | +25+25+25 | 58.34 ± 2.96 | 76.53 | CRV | CA | OCT | 0.001936 | CRV | CA | OCT |
| 5.75E-09 | 8.61E-09 | 1.24E-09 | 0.000065 | 0.000004 | 0.000005 | |||||
Inhibition of relative luminescence of ORco responses upon OA induction (100 μM) in the presence of low concentrations of selected antagonists and mixtures thereof (three repeat experiments, each in triplicate). Statistically significant differences, between mixtures and single compounds, are considered those with Pr(<[0.05]).
+, in addition to 100 μM OA; CA, cumin alcohol; CRV, carvacrol; DEET, N,N-diethyl-3-methylbenzamide; OCT, (2E,4E)-2,4-octadienal.
a
Fifty μM single compound concentration for two-compound mixtures, 75 μM single compound concentration for three-compound mixtures.