Table 1.
Kinetic parameters of TrtA constructs
| Variant | pHopt | KM (μM) | kcat (1/s) | kcat/KM (M−1s−1) |
|---|---|---|---|---|
| TrtA | 6.5 | 21 ± 3 | 11 ± 1 | 5.3 ± 1.0 × 105 |
| +Cleaved His-tag | 8 | 15 ± 3 | 8.8 ± 0.4 | 6.1 ± 1.6 × 105 |
| CtermDelΔ7+Cleaved His-tag | 8 | 16 ± 2 | 10 ± 1 | 6.3 ± 1.3 × 105 |
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