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Kinetic parameters of BiuHs for biuret and triuret
| Enzyme | Substrate | KM (μM) | kcat (1/s) | kcat/KM (M−1s−1) | Note |
|---|---|---|---|---|---|
| RhdBiuH | Biuret | 95 ± 26 | 1.5 ± 0.1 | 7.0 ± 2.0 × 103 | |
| Triuret | >1000 | 0.14a | 1.7 ± 0.4 × 102 | ||
| BiuH | Biuret | 23 ± 4 | 4.0 ± 0.2 | 1.7 ± 0.3 × 105 | Cameron et. al |
| Triuret | >1000 | 0.0044a | n.m | n.m - not measured |
Saturation kinetics were not achieved and activity was measured at 1 mM triuret RhdBiuH—Rhodococcus sp. Mel BiuH; BiuH—Rhizobium leguminosarum b.v 3841 BiuH.
