Specific apolipoproteins predominate in calcific aortic valve disease (CAVD) tissues.A, hierarchical cluster analysis (multigroup comparison; q ≤ 0.3) of the CAVD disease stage proteome (n = 9 donors; N = 1238 proteins with three or more unique peptides). Inset, is a zoomed view of apolipoproteins enrichment in CAVD tissue. B, sum-normalized apolipoprotein abundances across CAVD stages from the global proteomics data (upper panel; n = 9 donors) and from the parallel-reaction monitoring data (lower panel; n = 5 donors; averaged sum-normalized signal of one to three peptides per donor). C, fold change of gene expression of apolipoproteins between fibrotic and calcific or nonfibrotic/noncalcific and CAVD tissues from RNA-Seq. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, one-way ANOVA with Bonferroni multiple comparison test or Kruskal–Wallis test with Dunn's multiple comparison test for nonparametric data. C, calcific tissue; F, fibrotic tissue; N, nonfibrotic/noncalcific tissue.