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Aortic valve apolipoprotein localization and association with calcification. Negative control 1 and 2: staining without primary antibody, but horseradish peroxidase–conjugated antimouse and anti-rabbit secondary antibodies; representative immunohistochemistry images for apolipoproteins on human aortic valve tissue in the fibrosa layer (top panel, fibrosa facing up), around calcification (middle panel), and on nondiseased controls. The scale bar represents 100 μm.
