Figure 3.

Tissue apolipoprotein distribution.A, coimmunofluorescence for apoJ and vimentin (left panel) and apoJ and αSMA (right panel). B, coimmunofluorescence for apoD and CD68; yellow arrows indicate double-positive cells. The scale bar represents 100 μm. Observations were confirmed in three independent donors. C, immunofluorescence for apoC-III, vimentin (valvular interstitial cells), and calcification (Osteosense 680; near-infrared calcium tracer [Perkin Elmer, USA]) on fibrotic and calcific human aortic valve sections. D, oil red O staining of calcific human aortic valve tissue and apoC-III immunohistochemistry on serial sections identifying areas of lipid-poor apoC-III. αSMA, α-smooth muscle actin; DAPI, 4′,6-diamidino-2-phenylindole.