Figure 4.

ApoC-III is a contributor to valvular interstitial cell calcification.A, representative alizarin red staining of valvular interstitial cells incubated with normal media (NM), procalcifying media (PM), PM with 100 μg/ml human apoC-III (PM + apoC-III), and PM with 100 μg/ml human apoA-I (PM + apoA-I) at day 7 after first incubation; and alizarin red absorbance quantification (n = 4 independent donors, all passage 2). B, representative alizarin red staining of valvular interstitial cells incubated with NM, PM, PM + apoC-III, and PM + apoA-I at day 14 after first incubation; and alizarin red absorbance quantification (n = 4 independent donors, all passage 2).