Table 2.
RNA-Seq data for select glycan processing genes from three cell lines reporting the change in expression relative to primary human NK cells
| Gene | HEK293F | NK92 | YTS | Enzyme product |
|---|---|---|---|---|
| B3GNT7 | − − − | − − | − − − | β1-3GlcNAc in LacNAc repeats |
| B3GNT8 | n.s. | n.s. | − − | β1-3GlcNAc in LacNAc repeats |
| B4GALT2 | ++ | ++ | ++ | Branch β1-4Gal |
| B4GALT1 | − − | − | − | Branch β1-4Gal |
| FUT9 | +++ | ++ | n.s. | Branch (α1-3/4)Fuc |
| FUT4 | n.s. | − − | − − − | Branch (α1-3/4)Fuc |
| FUT5 | n.s. | − − | n.s. | Branch (α1-3/4)Fuc |
| FUT7 | − − | n.s. | ++ | Branch (α1-3/4)Fuc |
| MAN2A2 | − | − − − | n.s. | GlcNAc Man3 glycoform |
| MGAT4A | − | − − − | − − | Branch (β1-4GlcNAc to α1-3Man) |
| MGAT5 | − − | n.s. | − − − | Branch (β1-6GlcNAc to α1-6Man) |
| MGAT3 | ++ | n.s. | n.s. | Bisecting GlcNAc |
| MGAT5B | ++ | n.s. | ++ | Branch (β1-6GlcNAc to α1-6Man) |
| MGAT1 | − | n.s. | n.s. | Branch (GlcNAc Man5) |
| ST3GAL6 | ++ | ++ | − − | α2,3 Neu5Ac |
| ST6GAL1 | − − | − − | − | α2,6 Neu5Ac |
“−” indicates a decrease, “+” an increase, and “n.s.” is not significant at p-adj ≤ 0.05.
Complete data with fold change and significance values are shown in Table S1.