Table 2.
Kinetic parameters of reactions catalyzed by rhGFAT2-his
| Type of activity | Substrate(s) | Km (Gln) (mM) | Km (Fru-6P) (mM) | kcat (min−1) |
|---|---|---|---|---|
| Aminohydrolyzing activity | w/o Fru-6P | 0.820 ± 0.335 | – | 0.021 ± 0.003 |
| w/Fru-6P | 1.814 ± 1.158 | – | 0.079 ± 0.016 | |
| Isomerase activity | w/o Gln | – | 0.711 ± 0.170 | 0.322 ± 0.022 |
| GlcN-6P synthetic activity | Fru-6P | – | 0.957 ± 0.502a | 0.032 ± 0.007a |
| Gln | 0.763 ± 0.332a | – | 0.040 ± 0.008a |
a
The kinetic parameters for synthase activity were generated through Michaelis–Menten fitting, therefore must be considered as apparent values.