Figure 6.

Erythrocytes export NBD-S1P, but not TMR-S1P, in an Mfsd2b-dependent manner. A, B, TLC analysis of WT and Mfsd2b KO erythrocytes after incubation with 2.5 μM NBD-sphingosine in “continuous” assays. C, quantification of NBD-Sph and NBD-S1P bands from A and B. n = 3 for each genotype. D, E, TLC analysis of WT and Mfsd2b KO erythrocytes after incubation for 1 h with 2.5 μM TMR-sphingosine in “continuous” assays. F, quantification of TMR-Sph and TMR-S1P bands from D and E. In these experiments, NBD-sphingosine and TMR-sphingosine were used as the substrates for synthesis of the S1P analogs. Each dot represents one animal. Data are means and SD, ∗p < 0.05, ∗∗p < 0.001. p Value was calculated using unpaired i-test. ns, not significant.