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. 2020 Dec 24;296:100213. doi: 10.1074/jbc.RA120.015502

Figure 1.

Figure 1

Knockdown of KDM2B antagonized TGF-β-induced EMT phenotypes in A549 lung cancer and Panc1 pancreatic cancer cells.A–B, knockdown of KDM2B affected the changes in expression of EMT-related marker genes induced by TGF-β. QRT-PCR was performed to detect the expression of CDH1, VIM, FN1, ZEB1, ZEB2, SNAI1, SNAI2, CDH2, miR200a, and CGN in the control or KDM2B knockdown (KD) A549 (A) and Panc1 (B) cells with or without TGF-β for 24 h (n = 3) (∗∗p < 0.01; ∗p < 0.05; ns, not significant). C–D, immunoblotting of E-cadherin, Fibronectin, Vimentin, phosphorylated SMAD3 (P-SMAD3), and GAPDH proteins using the corresponding antibodies in A549 (C) and Panc1 (D) cells. E–F, KDM2B knockdown inhibited TGF-β-induced morphological changes of A549 (E) and Panc1 (F) cells. The cells were stained with crystal violet (upper), with anti-E-cadherin antibody and DAPI (middle), or with TRITC-phalloidin and DAPI (lower). Scale bars: 10 μm. G–H, KDM2B knockdown inhibited TGF-β-dependent increase of migrated cells (G) or invaded cells (H) through the filter. A549 and Panc1 cells that migrated or invaded through the filter were fixed, stained, and counted (n = 8) (∗∗p < 0.01; ns, not significant).