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. 2020 Dec 24;296:100213. doi: 10.1074/jbc.RA120.015502

Figure 2.

Figure 2

Overexpression of KDM2B repressed the expression of several epithelial marker genes but did not lead to complete EMT phenotypes in A549 and Panc1 cells.A–B, QRT-PCR analysis of CDH1, VIM, FN1, ZEB1, ZEB2, SNAI1, SNAI2, CDH2, miR200a, and CGN in A549 (A) and Panc1 (B) cells infected with the control retrovirus or the retrovirus expressing wild-type KDM2B (WT) with or without TGF-β for 24 h (n = 3) (∗∗p < 0.01; ∗p < 0.05; ns, not significant). C–D, immunoblotting of E-cadherin, Fibronectin, Vimentin, and GAPDH proteins in A549 (C) and Panc1 (D) cells with KDM2B overexpression. E–F, cell morphological changes induced by KDM2B overexpression in A549 (E) and Panc1 (F) cells. The cells were stained similarly in the case of Figure 1. Scale bars: 10 μm. G–H, cell migration (G) or cell invasion (H) activities of A549 and Panc1 cells with KDM2B overexpression (n = 8) (∗∗p < 0.01).