Figure 3.

KDM2B influenced the regulation of histone H2A ubiquitination and H3 methylation on the regulatory regions of several epithelial marker genes in A549 cells. A549 cells were infected with the control retrovirus or the retrovirus expressing wild-type KDM2B (WT) with or without TGF-β treatment. ChIP analyses of H2AK119Ub, KDM2B, H3K27me3, and EZH2 on the regulatory regions of CDH1 (A), miR200a/b (B), CGN (C), and GAPDH genes (D) in A549 cells are shown. The occupancies of ubiquitinated histones, KDM2B, methylated histones or EZH2 proteins on the regions were analyzed by quantitative PCR. Percentage enrichment over input chromatin DNA was presented (n = 3) (∗∗p < 0.01; ∗p < 0.05).