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. 2020 Dec 24;296:100213. doi: 10.1074/jbc.RA120.015502

Figure 4.

Figure 4

Combined knockdown of RING1A and RING1B showed similar inhibitory effects with KDM2B knockdown on TGF-β-dependent transcriptional regulation during EMT.A–B, combined knockdown of RING1A and RING1B affected the changes in expression of EMT-related marker genes induced by TGF-β. QRT-PCR was performed to detect the expression of CDH1, VIM, FN1, ZEB1, ZEB2, SNAI1, SNAI2, CDH2, miR200a, and CGN in the control, KDM2B, or RING1A/RING1B knockdown (KD) A549 (A) and Panc1 (B) cells with or without TGF-β for 24 h (n = 3) (∗∗p < 0.01; ∗p < 0.05; ns, not significant). C–F, ChIP analyses of H2AK119Ub, KDM2B, H3K27me3, and EZH2 on the regulatory regions of CDH1 (C), miR200a/b (D), CGN (E), and GAPDH genes (F) in A549 cells with KDM2B or RING1A/RING1B knockdown (n = 3) (∗∗p < 0.01; ∗p < 0.05; ns, not significant).