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. 2020 Dec 24;296:100213. doi: 10.1074/jbc.RA120.015502

Figure 5.

Figure 5

Overexpression of KDM2B mutants showed different effects in the expression of several epithelial marker genes through the regulation of histone H2A ubiquitination and H3 methylation.A–B, QRT-PCR analysis of CDH1, VIM, FN1, ZEB1, ZEB2, SNAI1, SNAI2, CDH2, miR200a, and CGN in A549 (A) and Panc1 (B) cells infected with the control retrovirus or the retrovirus expressing wild-type KDM2B (WT), JmjC domain mutant (JmjC Mut) or CXXC motif mutant (CXXC Mut) with or without TGF-β for 24 h (n = 3) (∗∗p < 0.01; ∗p < 0.05). C–D, immunoblotting of E-cadherin, Fibronectin, Vimentin, and GAPDH proteins in A549 (C) and Panc1 (D) cells with wild-type and mutant KDM2B overexpression. E–H, ChIP analyses of H2AK119Ub, KDM2B, H3K27me3, and EZH2 on the regulatory regions of CDH1 (E), miR200a/b (F), CGN (G), and GAPDH genes (H) in A549 cells with wild-type and mutant KDM2B overexpression (n = 3) (∗∗p < 0.01; ∗p < 0.05; ns, not significant).