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. 2020 Dec 24;296:100213. doi: 10.1074/jbc.RA120.015502

Figure 6.

Figure 6

Knockdown of EED, one of the core components of PRC2, showed inhibitory effects on TGF-β- or KDM2B- dependent expression changes of EMT-related marker genes during EMT.A–B, QRT-PCR analysis of CDH1, VIM, FN1, ZEB1, ZEB2, SNAI1, SNAI2, CDH2, miR200a, and CGN in the control or EED knockdown (EED shRNA#1) A549 (A) and Panc1 (B) cells coinfected with the control retrovirus or the retrovirus expressing wild-type KDM2B (WT) with or without TGF-β for 24 h (n = 3) (∗∗p < 0.01; ∗p < 0.05; ns, not significant). C–F, ChIP analyses of H2AK119Ub, KDM2B, H3K27me3, and EZH2 on the regulatory region of CDH1 (C), miR200a/b (D), CGN (E), and GAPDH genes (F) in the control or EED knockdown A549 cells with wild-type KDM2B overexpression (n = 3) (∗∗p < 0.01; ∗p < 0.05; ns, not significant).