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Sulfur-detected chromatograms of cytosol FTSs and standards. A–B, two batches of WT cytosol FTS. C–D, two batches of vma2Δ cytosol FTS. E–J, standards with areas normalized to indicate peak position. E, Na2S, F, cysteine, G, methionine, H, reduced glutathione, I, MgSO4, J, oxidized glutathione. The intense peak at ca. 20 ml in A–D is due to MES buffer (10 mM). FTS, flow-through solution.
