Table 1.

Metal concentrations in WT and Vma2Δ_W whole cells and cytosol fractions

Sample	n	Fe	Cu	Mn	Zn	P
WT pH 7	3	200 ± 30	100 ± 40	27 ± 8	500 ± 200	190 ± 10
WT pH 6	4	320 ± 40	100 ± 100	31 ± 3	1300 ± 500	500 ± 100
WT pH 5	4	300 ± 40	78 ± 5	60 ± 20	900 ± 100	500 ± 100
WT pH 4	3	260 ± 20	150 ± 10	44 ± 2	470 ± 60	280 ± 20
WT pH 3	4	190 ± 40	130 ± 10	18 ± 6	840 ± 40	210 ± 30
vma2Δw pH 5	4	250 ± 40	240 ± 50	10 ± 1	193 ± 6	170 ± 40
vma2Δw pH 4	2	128 ± 3	220 ± 20	7 ± 4	130 ± 20	67 ± 9
vma2Δw pH 3	5	160 ± 40	340 ± 40	2 ± 1	230 ± 20	104 ± 7
WT cytosol	2	270 ± 50	100 ± 7	25 ± 1	800 ± 100	490 ± 40
vma2Δw cytosol	4	200 ± 70	280 ± 70	8 ± 2	250 ± 80	170 ± 10
WT cytosol (1 μM Fe)	2	230 ± 20	130 ± 20	11 ± 3	340 ± 40	300 ± 30
WT FTS (1 μM Fe)	2	66 ± 2	13 ± 4	7 ± 2	130 ± 20	170 ± 6

Cells were grown on MM supplemented with 40 μM iron citrate (unless specified otherwise) and 10 μM CuSO₄. Samples were whole cells unless otherwise indicated. n, number of independent replicates. Concentrations are in μM except for P which is in mM. Reported cytosol and FTS concentrations are measured values after being multiplied by a 14× dilution factor.

FTS, flow-through solution; MM, minimal medium.