Figure 3.

Dephosphorylation of the myosin RLC and ELC increases the myosin SRX population. Scatter plots showing the differences between untreated (with significant levels of phosphorylated ELC and RLC; Fig. S3B in Supporting information) and dephosphorylated myosin samples for (A) A_slow, (B) k_fast, and (C) k_slow. Parameters are reported on an absolute scale (amplitudes in % and rates in s^-1). Statistical differences were based on a two-tailed t-test (∗∗p < 0.01; ∗∗∗p < 0.001). Data are expressed as the mean ± SEM (n ≥ 4 for each). ELC, essential light chain; RLC, regulatory light chain; SRX, super-relaxed.