Figure 2.

hPAT1 expression in oocytes detected by immunohistochemistry using anti-hPAT1 polyclonal antibody staining. (A) and (C), hPAT1 expression (red) was detected using anti-hPAT1 antibody on the plasma membranes of oocytes (C) injected with hPAT1 cRNA. (B) and (D), negative control (injected with water). (C-D) simultaneous immunostaining (green) with the plasma membrane marker Ca²⁺ ATPase (PMCA) was performed. The outer surfaces of the oocytes are indicated by blue arrows. DIC, differential interference contrast. Scale bar indicates 100 μm.