Figure 6.

Relative expression levels of PAT1 and DMT1,⁵⁹Fe and NA contents in various sections of mouse intestine. (A) mouse small intestine was divided into ten sections (sections numbered 1–10) that were defined as the duodenum (section 1), proximal jejunum (sections 2–5), distal jejunum (sections 6–7), and ileum (sections 8–10). (B) mRNA levels of PAT1, (C) DMT1 as determined by qRT-PCR in the duodenum (section. 1), proximal jejunum (sections 2–5), and distal jejunum (sections 6–7). The relative expression levels were calculated by setting the average values obtained for section 1 to 7 (n = 3–4, ∗∗p < 0.01, ∗p < 0.05). Uptake of ⁵⁹Fe was determined by radioactivity measurements in the upper intestine sections 1 to 7 30 min after oral administration of NA-⁵⁹Fe(II) (D) or ⁵⁹Fe(II) (E) (n = 4, ∗p < 0.05). F, amounts of NA obtained from each section of the small intestine. Quantification of NA levels in these extracts was performed by quantitative analysis with LC-MS after FMOC conversion. (n = 4, ∗p < 0.05).