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RT-PCR analysis of Pseudomonas aeruginosa hasA, hasR, and phuS. PA14 and PAO1 were grown in the absence or presence of CP (10 μM) in CDM. mRNA levels were normalized to oprf, and the fold change relative to the untreated condition for PA14 hasA (A) and hasR (B), and PAO1 phuS (C) is presented (N = 3, ∗p < 0.05, ∗∗p < 0.01). CDM, chemically defined medium; CP, calprotectin.
