Figure 3.

Heme allows Pseudomonas aeruginosa to prevent depletion of cellular iron by CP.A, cell-associated iron in P. aeruginosa PAO1 grown in CDM or iron-depleted CDM in the absence or presence of CP (10 μM) and heme (5 μM) (N = 5, ∗p < 0.05). B and C, cell-associated iron for P. aeruginosa PA14 grown in CDM (B) or Tris:TSB (C) in the absence or presence of CP-Ser (10 μM) and heme (5 μM) (N = 5, ∗p < 0.05, ∗∗p < 0.01). D, cell-associated Fe for P. aeruginosa PAO1 ΔhasRphuR grown in CDM in the absence or presence of CP-Ser (10 μM) and heme (5 μM) (N = 5, ∗∗p < 0.01). E, cell-associated ratio of ⁵⁶Fe:⁵⁷Fe in P. aeruginosa PAO1 grown in CDM in the absence or presence of CP (10 μM) and heme (5 μM) (N = 8, ∗∗p < 0.01). CDM, chemically defined medium; CP, calprotectin.