Figure 7.

Heme protectsStaphylococcus aureusfrom depletion of cellular iron and induction of iron starvation by CP. A, cell-associated iron for S. aureus USA300 JE2 grown in LB medium in the absence or presence of CP (20 μM) and heme (5 μM) (N = 5, ∗p < 0.05, ∗∗ p < 0.01). B, the CAS assay for S. aureus USA300 JE2 supernatants from cultures grown in LB medium in the absence or presence of CP (20 μM) and heme (5 μM). A₆₃₀ measurements were normalized to culture absorbance at 600 nm (N = 3, ∗p < 0.05, ∗∗p < 0.01). CAS, chrome azurol S; CP, calprotectin.