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. 2021 Feb 5;296:100386. doi: 10.1016/j.jbc.2021.100386

Figure 2.

Figure 2

Sphingosine-1 phosphate mediates its effects on trophoblast differentiation of hESCs through its receptors. A, confocal images of CTB from 6-day treatment of H9 hESCs using D-erythro-dihydrospingosine-1-phosphate (dhS1P), CYM5442 (S1PR1 agonist), CYM5220 (S1PR2 agonist), and CYM5541 (S1PR3 agonist), staining for CDX2, GATA3, P63, and TEAD4. Nuclei were stained with DAPI. B, confocal images of STB from 14-day treatment of H9 hESCs using dhS1P, CYM5442, CYM5520, and CYM5541 during initial 6-day treatment, staining for KRT7 and hCG. Nuclei were stained with DAPI. C, confocal images of EVTs from 12-day treatment of H9 hESCs using dhS1P, CYM5442, CYM5220, and CYM5541 during initial 6-day treatment, staining for HLA-G and VE-Cadherin. Nuclei were stained with DAPI. The scale bars represent 100 μm for all images. CTB, cytotrophoblast; DAPI, 4′,6-diamidino-2-phenylindole; EVT, extravillous trophoblast; hESC, human embryonic stem cell; STB, syncytiotrophoblast.