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. 2021 Feb 5;296:100386. doi: 10.1016/j.jbc.2021.100386

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© 2021 THE AUTHOR(S)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Transcriptome analysis confirms equivalence of hESC-derived and primary TS cells and reveals differences between hPSC-TS and hPSC-TS^CDX2. A, principal component analysis of transcriptome data on H1 and H9 hESCs, H1 and H9 hESCs after 3 days treatment, H1 and H9 hESC-derived hPSC-TS^CDX2 cultured in TM4, H1 and H9 hESC-derived hPSC-TS and placenta-derived TS #1 (CT30) and TS #2 (CT29) cultured in TSCM. B, spearman correlation coefficients for comparison between hESCs (H1 and H9), hESC after 3 days treatment (H1 and H9), hESC-derived hPSC-TS^CDX2 cultured in TM4, hESC-derived hPSC-TS (H1 and H9) and placenta-derived TS #1 (CT30) and TS #2 (CT29) cultured in trophoblast stem cell medium (p < 0.00001). C, hierarchical clustering analysis of transcriptome data from H1 and H9 hESC-derived hPSC-TS and placenta-derived TS #1 (CT30) and TS #2 (CT29). Four biological replicates (i.e., cells from different passages) were used. D, relative expression of trophectoderm-associated markers CDX2 and HAND1 and villous cytotrophoblast-associated marker TP63 in hESC-derived hPSC-TS^CDX2 and hPSC-TS (H1 and H9) (∗q < 0.001). hESC, human embryonic stem cell; hPSC, human pluripotent stem cell; TS, trophoblast stem.