Table 1.
Key resources
| Reagent or resource | Source | Identifier |
|---|---|---|
| hPSC cell lines | ||
| H1 hESCs | Wicell | RRID: CVCL_9771 |
| H9 hESCs | Wicell | RRID: CVCL_9773 |
| SC102A-1 hiPSCs | Systems Biosciences | RRID: CVCL_IT66 |
| Antibodies and staining reagents | ||
| Anti-KRT7 | Santa Cruz Biotechnology | Cat#sc-23876, RRID:AB_2265604 |
| Anti-KRT7 | Cell Signaling Technologies | Cat# 4465, RRID:AB_11178382 |
| Anti-hCG | Abcam | Cat# ab9582, RRID:AB_296507 |
| Anti-hCG | Abcam | Cat# ab9376, RRID:AB_307221 |
| Anti-P63 | Cell Signaling Technologies | Cat# 13,109, RRID:AB_2637091 |
| Anti-GATA3 | Cell Signaling Technologies | Cat# 5852, RRID:AB_10835690 |
| Anti-TFAP2C | Cell Signaling Technologies | Cat# 2320, RRID:AB_2202287 |
| Anti-YAP | Cell Signaling Technologies | Cat# 4912, RRID:AB_2218911 |
| Anti-TEAD4 | Abcam | Cat# ab58310, RRID:AB_945789 |
| Anti-CDX2 | Abcam | Cat# ab76541, RRID:AB_1523334 |
| Anti-VE-Cadherin | Cell Signaling Technologies | Cat# 2500, RRID:AB_10839118 |
| Anti-HLA-G | Abcam | Cat# ab52455, RRID:AB_880552 |
| Anti-Syncytin | Santa Cruz Biotechnology | Cat# sc-50369, RRID:AB_2101536 |
| Rabbit Polyclonal IgG | R&D Systems | Cat# AB-105-C, RRID:AB_354266 |
| Rabbit XP IgG | Cell Signaling Technologies | Cat# 3900, RRID:AB_1550038 |
| Mouse IgG1 | Abcam | Cat# ab18447, RRID:AB_2722536 |
| Mouse IgG2a | Abcam | Cat# 554126, RRID:AB_479661 |
| Alexa Fluor 488–conjugated anti-rabbit IgG | Thermo Fisher Scientific | Cat# A-11034, RRID:AB_2576217 |
| Alexa Fluor 647–conjugated anti-rabbit IgG | Thermo Fisher Scientific | Cat# A-21052, RRID:AB_2535719 |
| DAPI | R&D Systems | Cat#5748 |
| CellMask deep red plasma membrane stain | Invitrogen | Cat#C10046 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| TrypLE | Thermo Fisher Scientific | Cat#12604013 |
| Vitronectin | Thermo Fisher Scientific | Cat#A14700 |
| Laminin 521 | Stem Cell Technologies | Cat#77003 |
| Human FGF-10 | Stem Cell Technologies | Cat#78037 |
| TeSR-E8 | Stem Cell Technologies | Cat#05990 |
| TeSR-E7 | Stem Cell Technologies | Cat#05914 |
| TeSR-E6 | Stem Cell Technologies | Cat#05946 |
| ReLeSR | Stem Cell Technologies | Cat#05872 |
| Sphingosine-1-phosphate | Tocris | Cat#1370 |
| D-erythro-dihydrosphingosine-1-phosphate | Abcam | Cat#ab141750 |
| SB431542 | Tocris | Cat#1614 |
| BMP4 | Thermo Fisher Scientific | Cat#PHC9534 |
| CYM5442 hydrochloride | Tocris | Cat#3601 |
| CYM5520 | Tocris | Cat#5418 |
| CYM5541 | Tocris | Cat#4897 |
| Y-27632 dihydrochloride | Tocris | Cat#1254 |
| EGF | R&D Systems | Cat#236-EG |
| Doxycycline hyclate | Tocris | Cat#4090 |
| Puromycin dihydrochloride | Tocris | Cat#4089 |
| Activin A | R&D Systems | Cat#338-AC |
| Greiner Bio-one Cell View glass plates | Greiner Bio-one | Cat#627965 |
| 4% Paraformaldehyde in PBS | Thermo Fisher Scientific | Cat#R37814 |
| Triton X-100 | Sigma | Cat#T8787 |
| PBS w/o CaMg | Sigma | Cat#D5773 |
| PBS w/CaMg | Sigma | Cat#D8662 |
| Human IgG | Immunoreagents | Cat#Hu-003-C |
| BSA | Fisher Scientific | Cat#BP9703 |
| 10% BSA fatty acid free in PBS | Sigma | Cat#A1595 |
| VPA | Sigma | Cat#P6273 |
| A83–01 | Tocris | Cat#2939 |
| 2-mercaptoethanol | Sigma | Cat#M3148 |
| FBS | Thermo Fisher Scientific | Cat#16141–061 |
| DMEM/F12 | Thermo Fisher Scientific | Cat#11320033 |
| ITS-X | Thermo Fisher Scientific | Cat#51500–056 |
| L-ascorbic acid | Sigma | Cat#A8960 |
| Pen/Strep | Thermo Fisher Scientific | Cat#15140122 |
| Forskolin | Tocris | Cat#1099 |
| Neuregulin | Cell Signaling Technologies | Cat#5218SC |
| Matrigel | Corning | Cat#354234 |
| KSR | Thermo Fisher Scientific | Cat#10828028 |
| Trizol Reagent | Thermo Fisher Scientific | Cat#15596018 |
| DEPC | Sigma | Cat#95284 |
| Baseline Zero DNAase Kit | VWR | Cat#76081–624 |
| Oligo-dT | IDT | Cat#51–01–15–07 |
| dNTP mix | Thermo Fisher Scientific | Cat#10297018 |
| Superscript II RT | Thermo Fisher Scientific | Cat#18064014 |
| SYBR Green Supermix | Bio-rad | Cat#1725272 |
| Methanol | Fisher Scientific | Cat#A412–500 |
| Acetone | Fisher Scientific | Cat#A18–500 |
| Critical Commercial Kits | ||
| GeneJET RNA Purification Kit | Thermo Fisher Scientific | Cat#K0731 |
| Oligonucleotides | ||
| qPCR Primers | IDT | Methods S1 for primer sequences |
| Software and Algorithms | ||
| R (v3.6.0) | http://www.R-project.org/ | N/A |
| DESeq2 package (v1.22.2) | ||
| PCR package (v1.2.2) | ||
| SAS Software | N/A | |
| Zeiss Zen Software | https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html | N/A |