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. 2021 Mar 7;27:e928503-1–e928503-8. doi: 10.12659/MSM.928503

Table 1.

Primer sequences of the studied LEPR SNPs.

SNP Alleles MAF (1000g_CHBS) iMLDR primers sequences
rs1137101 G/A 0.13 F: 5′-TGTGCCAACAGCCAAACTCAAC-3′
R: 5′-AGAAGCCACTCTTAATACCCCCAGT-3′
rs1938496 C/T 0.21 F: 5′-TGCTTGCCATTATGAAGAACAGC-3′
R: 5′-CAAGAATCTGTGGGCAAAAAGCTC-3′
rs6588147 G/A 0.16 F: 5′-CCAGCCACAAAGAGCAAGGTAGA-3′
R: 5′-GGAGGGTCAAGAGGCACTGAGA-3′
rs7555955 G/A 0.20 F: 5′-TGTTTTTCCAAAAGACCTTTGACCA-3′
R: 5′-TCACGGGATGTTATGTTTCTATTTTGA-3′

LEPR – leptin receptor; SNP – single nucleotide polymorphism; MAF – minor allele frequency; 1000g_CHBS – minor allele frequencies from HapMap of Han Chinese in Beijing, China(CHB) or Human Genome Project; iMLDR – improved multiple ligase detection reaction; F – forward; R – reverse.