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FRET analysis of Ub–β-catenin. The real-time kinetic reaction was carried out as described in Experimental procedures section. The inset shows gel images of the final reaction products detected by scanning with Typhoon FLA9500 for both iFluor 647 and iFluor 555, respectively. Approximately 28% of input Ub (I647)–β-catenin was converted into Ub2–β-catenin. Ub, ubiquitin.
